5k Mouse Splenocytes

Universal 5' Gene Expression dataset analyzed using Cell Ranger 9.0.0

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

Splenocytes from a C57BL/6 mouse strain were obtained by 10x Genomics from BioIVT.

Gene Expression, VDJ-T, and VDJ-B libraries were generated as described in the Chromium GEM-X Single Cell 5' Reagent Kits v3 User Guide (CG000733) and sequenced on an Illumina NovaSeq 6000 with approximately 37,000 read pairs per cell for the Gene Expression library, 30,000 read pairs per cell for the VDJ-T library and 14,000 read pairs for the VDJ-B library.

Paired-end, dual indexing libraries were sequenced with the following configuration:

28 cycles for Read 1
10 cycles for i7
10 cycles for i5
90 cycles for Read 2

Libraries were analyzed using the cellranger multi pipeline.

See companion datasets

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.