5k Human PBMCs Stained with TotalSeq™-B Human TBNK Cocktail, Chromium NextGEM Single Cell 3'
Universal 3' Gene Expression dataset analyzed using Cell Ranger 8.0.0
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Peripheral blood mononuclear cells (PBMCs) from a healthy human male donor, aged 18-35, were obtained by 10x Genomics from AllCells.
2.5 million cells were stained with TotalSeq™-B Human TBNK Cocktail (BioLegend, Cat# 399902) following the manufacturer's guidance for antibody reconstitution and the 10x Genomics demonstrated protocol for Cell Surface Protein Labeling for Single Cell RNA.
Sequencing Protocols with Feature Barcode technology (CG000149, Rev D) were employed. After staining, cells were washed using the Wash Protocol 1 option.
Gene Expression and Cell Surface Protein libraries were generated as described in the Chromium Single Cell 3' Reagent Kits User Guide (v3.1- Dual Index) with Feature Barcoding technology for Cell Surface Protein User Guide (CG000317) and sequenced on an Illumina NovaSeq 6000, with approximately 75,000 read pairs per cell.
Paired-end, dual indexing:
- 28 cycles Read 1
- 10 cycles i7
- 10 cycles i5
- 90 cycles Read 2
The cellranger multi pipeline in Cell Ranger v8.0.0 was used to analyze libraries.
This dataset is licensed under the Creative Commons Attribution license.