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500 1:1 Mixture of Fresh Frozen Human (GM12878) and Mouse (A20) Cells (Next GEM v1.1)

Single Cell ATAC dataset analyzed using Cell Ranger ATAC 1.2.0

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Learn about Chromium analysis

1:1 mixture of human (GM12878) and mouse (A20) cells. This is a classic human-mouse mixture experiment to demonstrate single cell behavior.

Libraries were prepared following the Chromium Next GEM Single Cell ATAC Reagent Kits v1.1 (User Guide CG000209 Rev A). All GM12878:A20 mixture libraries (v1.1 chemistry vs. v1 chemistry at all cell loads) were prepared with the same nuclei preparation.

  • ~750 transposed nuclei were loaded. ~500 expected nuclei recovered. 251 human and 238 mouse nuclei were detected.
  • Sequenced on Illumina NovaSeq with approximately 31k read pairs per cell:
    • 50 bp Read 1
    • 8 bp i7 index (sample index)
    • 16 bp i5 (10x Barcode)
    • 49 bp Read 2

This dataset is licensed under the Creative Commons Attribution license.