5k Peripheral Blood Mononuclear Cells (PBMCs) from a Healthy Donor with a Panel of TotalSeq™-B Antibodies (Next GEM)

Universal 3' Gene Expression dataset analyzed using Cell Ranger 3.1.0

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

Peripheral blood mononuclear cells (PBMCs) from a healthy donor were stained with a panel of 31 TotalSeq™-B antibodies (BioLegend).

PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

Libraries were prepared following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 with Feature Barcode technology for Cell Surface Protein User Guide (CG000206 RevA).

5,527 cells were detected
Sequenced on Illumina NovaSeq with approximately 30,853 reads per cell
- 28 bp Read 1 (16 bp Chromium barcode and 12 bp UMI)
- 91 bp Read 2 (transcript)
- 8 bp i7 index (sample barcode)
run with --expect-cells=5000

This dataset is licensed under the Creative Commons Attribution license.