20k 1:1 Mixture of Human HEK293T and Mouse NIH3T3 cells, 5' HT v2.0

Universal 5' Gene Expression dataset analyzed using Cell Ranger 6.1.0

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

1:1 mixture of fresh frozen human HEK293T and mouse NIH3T3 cells obtained by 10x Genomics from ATCC were used in a classic experiment to demonstrate single cell behavior.

Libraries were generated from ~33,000 cells (19,613 cells recovered) as described in the Chromium Next GEM Single Cell 5' HT Reagent Kits v2 User Guide (CG000421) using the Chromium X and sequenced on an Illumina NovaSeq 6000 to a read depth of approximately 50,000 mean reads per cell.

Paired-end, dual indexing Read 1: 26 cycles (16 bp barcode, 10 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript).

Run with --expect-cells=20000.

This dataset is licensed under the Creative Commons Attribution license.