10k Human DTC Melanoma, Chromium NextGEM Single Cell 5'
Single Cell Immune Profiling dataset analyzed using Cell Ranger 8.0.0
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Dissociated tumor cells (DTCs) from melanoma tissue (female, age 50-60) were obtained by 10x Genomics from Discovery Life Sciences.
Gene Expression and VDJ libraries were generated following the guidelines in the Chromium Single Cell 5' Reagent Kits User Guide (v2 - Dual Index) (CG000331) and sequenced on an Illumina NovaSeq 6000 with approximately 60,000 read pairs per cell.
Paired-end, dual indexing:
- 28 cycles Read 1
- 10 cycles i7
- 10 cycles i5
- 90 cycles Read 2
The cellranger multi pipeline in Cell Ranger v8.0.0 was used to analyze libraries.
This dataset is licensed under the Creative Commons Attribution license.