10k Human DTC Melanoma, Chromium NextGEM Single Cell 3'
Universal 3' Gene Expression dataset analyzed using Cell Ranger 8.0.0
Visualize and explore data
Discover differentially expressed genes, visualize your favorite genes, and explore your data with our visualization software.
Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Dissociated tumor cells (DTCs) from melanoma tissue (female, age 50-60) were obtained by 10x Genomics from Discovery Life Sciences.
Gene Expression libraries were generated as described in the Chromium Single Cell 3' Reagent Kits User Guide (v3.1 - Dual Index) (CG000315) and sequenced on an Illumina NovaSeq 6000 with approximately 55,000 read pairs per cell.
Paired-end, dual indexing:
- 28 cycles Read 1
- 10 cycles i7
- 10 cycles i5
- 90 cycles Read 2
The cellranger multi pipeline in Cell Ranger v8.0.0 was used to analyze libraries.
This dataset is licensed under the Creative Commons Attribution license.