10k Human PBMCs, Multiome v1.0, Chromium Controller

Single Cell Multiome ATAC + Gene Expression dataset analyzed using Cell Ranger ARC 2.0.0

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Dataset overview

Output and supplemental files

Input files

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Human peripheral blood mononuclear cells (PBMCs) of a healthy male donor aged 30-35 were obtained by 10x Genomics from AllCells. Nuclei were isolated using the demonstrated protocol Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing (CG000365).

Gene Expression and ATAC libraries were generated from ~16,100 transposed nuclei (10,699 nuclei recovered) as described in the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide (CG000338 Rev E) using the Chromium Controller and sequenced on an Illumina NovaSeq 6000.

ATAC libraries were sequenced to ~55k read pairs per cell.

50 cycles Read 1
8 cycles i7 (sample index)
24 cycles i5 (10x barcode)
49 cycles Read 2.

Gene Expression libraries were sequenced to ~55k reads per cell.

28 cycles Read 1 (16 bp barcode, 12 bp UMI)
10 cycles i7 (sample index)
10 cycles i5 (sample index)
90 cycles Read 2 (transcript).

This dataset is licensed under the Creative Commons Attribution license.