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1k PBMCs from a Healthy Donor (v2 chemistry)

Universal 3' Gene Expression dataset analyzed using Cell Ranger 3.0.0

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Peripheral blood mononuclear cells (PBMCs) from a healthy donor (the same cells were used to generate pbmc_1k_v3, pbmc_10k_v3). PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

  • 996 cells detected
  • Sequenced on Illumina NovaSeq with approximately 77,000 reads per cell
  • 28bp read1 (16bp Chromium barcode and 10bp UMI, extra 2bp ignored), 91bp read2 (transcript), and 8bp I7 sample barcode
  • run with --expect-cells=1000

This dataset is licensed under the Creative Commons Attribution license.