This document provides guidance on preparation of Fresh Frozen tissue blocks and generating tissue slides. Demonstrated protocols for performing tissue staining (H&E and IF) are included.

Key companion documents for use with this document:

1. Visium HD Spatial Gene Expression Protocol Planner (CG000698)
2. Tissue Slide Alignment Instruction, Quick Reference Cards (CG000648)
3. Visium S3 for Tissue Slide Cassette Assembly Quick Reference Cards (CG000730)

For use with:

• Visium HD, Human Transcriptome, 6.5 mm, 4 rxns, PN-1000675
• Visium HD, Human Transcriptome, 6.5 mm, 16 rxns, PN-1000673
• Visium HD, Mouse Transcriptome, 6.5 mm, 4 rxns, PN-1000676
• Visium HD, Mouse Transcriptome, 6.5 mm, 16 rxns, PN-1000674
• Visium CytAssist Reagent Accessory Kit, PN-1000499