Protocols labeled "2.0" are compatible with Visium HD 6.5 mm and 11 mm slides and contain a "Featured" tag. For ongoing Visium HD 6.5 mm projects, please use original protocol versions.
This document provides best practices for handling FFPE tissue samples and generating tissue slides. Protocols for performing deparaffinization, staining (H&E and IF), and decrosslinking of tissue sections. Key companion documents for use with this document:
- Visium HD Spatial Gene Expression Protocol Planner (CG000698)
- Tissue Slide Alignment Instruction, Quick Reference Cards (CG000548)
- Visium Cassette S3 for Assembly & Disassembly (CG000730)
For use with:
- Visium HD, Human Transcriptome, 6.5 mm, PN-1000675 (4 rxns) / PN-1000673 (16 rxns)
- Visium HD, Mouse Transcriptome, 6.5 mm, PN -1000676 (4 rxns) / PN-1000674 (16 rxns)
- Visium CytAssist Reagent Accessory Kit, PN-1000499
- Dual Index Kit TS Set A, 96 rxns, PN-1000251