The Visium CytAssist Spatial Gene and Protein Expression workflow assays RNA levels and protein detection in intact formalin-fixed paraffin embedded (FFPE) tissue sections. It uses probes targeting the human whole transcriptome and oligonucleotide conjugated antibodies for detecting immune cell protein markers. Each Visium CytAssist Spatial Gene Expression Slide contains Capture Areas with barcoded spots that include oligonucleotides required to capture gene expression probes and antibody tags.

This document outlines the protocol for generating Visium CytAssist Spatial Gene Expression probe-based libraries and Visium CytAssist Spatial Protein Expression libraires from FFPE tissue sections.

For use with:

• Visium CytAssist Spatial Gene Expression for FFPE, Human Transcriptome, 6.5 mm, 4 rxns PN-1000520
• Visium CytAssist Spatial Gene Expression for FFPE, Human Transcriptome, 11 mm, 2 rxns PN-1000522
• Visium CytAssist Protein Core Reagents PN-1000603
• Human Immune Cell Profiling Panel, Small PN-1000607
• Visium CytAssist Reagent Accessory Kit, PN-1000499
• Visium CytAssist Tissue Slide Cassette, 4 pk, 6.5 mm PN-1000471
• Visium CytAssist Tissue Slide Cassette, 4 pk, 11 mm PN-1000472
• Dual Index Kit TS Set A, 96 rxns PN-1000251
• Dual Index Kit NT Set A, 96 rxns PN-1000242