The Chromium X Series offers a highly optimized approach to single cell studies and enables both low and high throughput experiments. All 10x Genomics Chromium Next GEM Single Cell assays are supported on Chromium X Series instruments. This Technical Note provides network connectivity guidelines for Chromium X Series instrument to enable easy updates and 10x Genomics Cloud connection to reduce unplanned instrument downtime, minimize run failures and sample loss, and help troubleshoot any connectivity issues.