Supported Thermal Cyclers

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Xenium Gene Expression.

Experimental Design

Experimental Design & Planning

The Xenium In Situ enables high-throughput subcellular mapping of 100s of RNA targets, revealing new insights into cellular structure and function. The platform includes a versatile and easy-to-use instrument, sensitive and specific chemistry, and a diverse menu of customizable panels.

Learn about the pre-designed Gene Expression panels and engage with 10x design experts to design, order, and manufacture Xenium Custom Genes.

Panel Design

For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Demonstrated Protocols for FFPE.

Tissue Prep for FFPE

For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Demonstrated Protocols for Fresh Frozen.

Tissue Prep for FF

Tissue Prep for Fresh Frozen

Prepare tissue sections on Xenium slides for on-instrument analysis by following our step-by-step User Guide/Protocols.

Assay

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Analyze your Xenium in situ data using our pipelines and visualization tools.

Software Analysis

Safety Data Sheets

Xenium Analyzer

In Situ遺伝子発現

Overview

User Guides

Workflow Documentation

Datasets

Videos

Service Providers

In Situ Gene Expression

<>{'NEW Xenium Cell Segmentation Add-On Kit User Guide. NEW Storage Conditions for Xenium slides. Refer to '}<Anchor href={'/support/in-situ-gene-expression/documentation/steps/assay/xenium-in-situ-gene-expression-probe-hybridization-ligation-amplification'}>{'Xenium In Situ Gene Expression - Probe Hybridization, Ligation & Amplication User Guide (CG000582 Rev F)'}</Anchor>{' for details.'}</>

What is In Situ Gene Expression?

Find resources, from protocols to free-to-use analysis tools. Workflow varies based on your sample type. Xenium In Situ Gene Expression currently supports both fresh frozen and FFPE samples.

Documentation

Access free, publicly available data from a number of different sample types generated with Xenium In Situ solutions.

Xenium In Situ Tissue Preparation

Xenium In Situ Assay Protocols - Best Practices

Find answers to common technical questions, from sample prep through data analysis, about In Situ workflows. 

Thermal Cycler	Part Number	Xenium v1	Xenium Prime
Analytik Jena Biometra TAdvanced 96 S †	846-x-070-251
Analytik Jena Biometra TAdvanced 96 SG †	846-x-070-241
Eppendorf Mastercycler X50s ‡	6311000010
Eppendorf Mastercycler X50a ‡	6313000018
BioRad PTC Tempo Deepwell	12015392
ThermoFisher VeritiPro ¶	A48141
VWR XT96 Gradient §	76452-153
MJ Research PTC-200 (discontinued)	05434-05		`-`
BioRad C1000 (discontinued)	1851197
ThermoFisher Veriti (discontinued)	4375786		`-`
Eppendorf Mastercycler Pro (discontinued)	950030010	`-`	`-`

Supported Thermal Cyclers

Supported thermal cyclers for Xenium workflows