Visium CytAssist Gene Expression Libraries of Post-Xenium Human Colon Cancer (FFPE)

Overview

This dataset is provided as part of the Technical Note: Post-Xenium In Situ Applications: Immunofluorescence, H&E, and Visium CytAssist Spatial Gene Expression (CG000709). Post-Xenium samples were compared to controls (samples not processed through the Xenium workflow) using 5 µm (FFPE) serial sections.

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Biomaterials

Human colon cancer samples (FFPE; adenocarcinoma stage IIA) were obtained from Avaden Biosciences.

Tissue preparation

Prior to the Visium CytAssist Spatial Gene Expression workflow, all sections were placed on Xenium slides. Two replicates were processed through the Xenium workflow prior to the Visium CytAssist workflow (post-Xenium). The other two replicates were treated as controls, not processed with the Xenium workflow.

FFPE human colon sections used for post-Xenium applications were generated using a custom gene panel designed for human colon gene expression profiling. For more details on methods, see page 13 of the Technical Note.

Library preparation & sequencing

Consult the Technical Note for details.

• Probe set: Human Whole Transcriptome
• Slides: 11 mm, V53M06-039 (Replicate 1), V53M06-063 (Replicate 2)
• Areas: A1 (control), B1 (post-Xenium)
• Sequencing instrument: Illumina NovaSeq 6000
• Sequencing configuration: 28 bp read 1 (16 bp Visium spatial barcode, 12 bp UMI), 90 bp read 2 (probe insert), 10 bp i7 sample index and 10 bp i5 sample index

Analysis

The resulting FASTQ files and image data were analyzed with Space Ranger v2.1 to produce the output files.

How to view data

To get started, download Loupe Browser to explore the Loupe Browser file, or read more about the other Space Ranger outputs.

This dataset is licensed under the Creative Commons Attribution license.