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PBMCs from Heparin-Treated Blood Collection Tubes Isolated via SepMate-Ficoll Gradient (5' v2 Chemistry)

Universal 5' Gene Expression dataset analyzed using Cell Ranger 6.1.0

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Donor Information: Healthy female

Isolation protocol: CG000392.RevA: Isolation of Leukocytes, Bone Marrow and Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing.

Whole transcriptome/Gene Expression libraries were generated as described in the Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) User Guide (CG000331 Rev A).

Sequencing data were processed using Cell Ranger, with web_summary.html and output files available in relevant tabs.

  • Sequencing instrument: Illumina NovaSeq 6000
  • Sequencing depth: 28,722
  • Sequencing configuration: Paired-end (28 X 90), single-indexed. Read 1: 28 cycles (16 bp barcode, 10 bp UMI); i7 index: 10 cycles; i5 index: 10 cycles; Read 2: 90 cycles (transcript).
  • Cells detected: 3,791
  • Median genes per cell: 1,580
  • Median UMIs per cell: 4,465

This dataset is licensed under the Creative Commons Attribution license.