PBMC from a Healthy Donor - No Cell Sorting (3k)

Cryopreserved human peripheral blood mononuclear cells (PBMCs) of a healthy female donor aged 25 were obtained by 10x Genomics from AllCells.

Nuclei were isolated as described in the Demonstrated Protocol- Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing (CG000365 Rev A).

Paired ATAC and Gene Expression libraries were generated from the isolated nuclei as described in the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression User Guide (CG000338 Rev A) and sequenced on Illumina Novaseq 6000 v1 Kit (Forward Strand Dual-Index Workflow).

User Guide: Chromium Next Gem Single Cell Multiome ATAC + Gene Expression User Guide (CG000338 Rev A)

Libraries generated:

• Chromium Single Cell Multiome ATAC library
• Chromium Single Cell Multiome Gene Expression library

Sequencing information:

• Sequencer: Novaseq 6000 v1 Kit (Forward Strand Dual-Index Workflow)
• Cycle numbers:
  • Gene Expression library: 28,10,10,90
  • ATAC library: 50,8,16,49

Key metrics:

• Estimated number of cells: 3,012
• ATAC Mean raw read pairs per cell: 27,483
• ATAC Median high-quality fragments per cell: 8,890
• ATAC Number of peaks: 77,242
• GEX Mean raw reads per cell: 56,540
• GEX Median genes per cell: 1,494
• GEX Median UMI counts per cell: 2,762
• Linked genes: 7,065
• Linked peaks: 32,116

This dataset is licensed under the Creative Commons Attribution license.