PBMCs of a Healthy Donor - 5' Gene Expression with a Panel of TotalSeq™-C Antibodies
Universal 5' Gene Expression dataset analyzed using Cell Ranger 3.0.0
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Peripheral blood mononuclear cells (PBMCs) of a healthy donor, purchased from AllCells (Catalog #: PB001), 87% viable by Trypan blue stain. PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).
The same cDNA was used to generate TCR (vdj_v1_hs_pbmc2_t) and B cell Ig (vdj_v1_hs_pbmc2_b) specific libraries.
Libraries were prepared following the Single Cell V(D)J Reagent Kits User Guide (CG000186 RevA).
- ~17,000 total cells loaded
- ~9,000 expected cells recovered (based on cell viability and capture efficiency)
- 8,258 cells detected
- 1,508 median genes per cell and 4,699 median UMI counts per cell detected
- Sequenced on an Illumina NovaSeq 6000 (NovaSeq software v1.4) with ~54,000 read pairs per cell
- 26bp read1 (including 16bp Chromium barcode and 10bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode read configuration for gene expression data; 28bp read1 (including 16bp Chromium barcode and 10bp UMI), 91bp read2 (Feature Barcode oligonucleotide), and 8bp I7 sample barcode read configuration for cell surface protein data
This dataset is licensed under the Creative Commons Attribution license.