Human Skin Preview Data (Xenium Human Skin Gene Expression Panel)

In Situ Gene Expression dataset analyzed using Xenium Onboard Analysis 1.6.0

This preview dataset was generated using a preliminary version of the Xenium Human Skin Gene Expression Panel and may be replaced with data generated using the final panel in the future.

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Dataset overview

Output and supplemental files

Input files

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Overview

Preview of Xenium In Situ Gene Expression data for adult human skin sections, using a development version of the Xenium Human Skin Gene Expression Panel.

Biomaterials

FFPE-preserved tissues were purchased from Avaden Biosciences (Melanoma).

Tissue preparation

Tissues were prepared following the Demonstrated Protocols Xenium In Situ for FFPE - Tissue Preparation Guide (CG000578) and Xenium In Situ for FFPE Tissues – Deparaffinization & Decrosslinking (CG000580). Additional guidance for sectioning skin samples is provided here.

Post-instrument processing followed the Demonstrated Protocol Xenium In Situ Gene Expression - Post-Xenium Analyzer H&E Staining (CG000613).

Gene panels

The Xenium Human Skin Gene Expression Panel (282 genes) was pre-designed by 10x Genomics. Genes were chosen to prioritize identification of keratinocytes, melanocytes, fibroblasts, Langerhans cells, vascular endothelial, immune cells and other cell types in human normal skin, melanoma, basal cell carcinoma, and squamous cell carcinoma.

Xenium Analyzer

The instrument run was performed following the Xenium Analyzer User Guide (CG000584). The on-instrument analysis was run with Xenium Onboard Analysis version 1.6.0.

Metric	Melanoma, pre-designed panel
Cells detected	106,980
Decoded transcripts per 100 µm²	137.7
Median transcripts per cell	163
Total high quality decoded transcripts	20,385,532
Region area (µm²)	23,221,129

How to view data

Interactively explore data with Xenium Explorer by downloading the Xenium Output Bundle (or Xenium Explorer subset) file. The subset bundle contains the experiment.xenium, morphology_mip.ome.tif, analysis_summary.html, cells.zarr.zip, cell_feature_matrix.zarr.zip, transcripts.zarr.zip, and analysis.zarr.zip files. See the Getting Started with Xenium Explorer page for more details. Follow these instructions to view the post-Xenium H&E image or image alignment file in Xenium Explorer.

This dataset is licensed under the Creative Commons Attribution license.