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750 Sorted Cells from Human Invasive Ductal Carcinoma, 3’ LT v3.1

Universal 3' Gene Expression dataset analyzed using Cell Ranger 6.0.0

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Invasive Ductal Carcinoma cells from a female donor aged 65 were obtained by 10x Genomics from Discovery Life Sciences.

Libraries were prepared following the Chromium Next GEM Single Cell 3' LT Reagent Kits v3.1 (Dual Index) User Guide (CG000399) and sequenced on Illumina NovaSeq 6000.

Sequencing Depth: 62,379 mean reads per cell

Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript)

Analysis Parameters Used: Run with --expect-cells=750

This dataset is licensed under the Creative Commons Attribution license.