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6k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

Universal 3' Gene Expression dataset analyzed using Cell Ranger 2.1.0

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1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells. This is a classic human-mouse mixture experiment to demonstrate single cell behavior.

  • 6,807 cells detected
  • Sequenced on Illumina Hiseq4000 with approximately 55,000 reads per cell
  • 26bp read1 (16bp Chromium barcode and 10bp UMI), 98bp read2 (transcript), and 8bp I7 sample barcode
  • Analysis run with --expect-cells=6000
  • Analysis run with reference version 1.2.0

This dataset is licensed under the Creative Commons Attribution license.