40k Mixture of Dissociated Tumor Cells from 4 Donors, Multiplexed Samples, 4 Probe Barcodes (Next GEM)

Dissociated tumor cells from four different cancers were obtained by 10x Genomics from Discovery Life Sciences:

• Bladder Cancer, Transitional Cell Carcinoma (Male, Age late 50s)
• Breast Cancer, Medullary Carcinoma (Female, Age late 50s)
• Ovarian Cancer, (Female, Age mid 40s)
• Kidney Cancer, Renal Cell Carcinoma (Female, Age mid 60s)

Cells were fixed for 1 hour at room temperature following the demonstrated protocol Fixation of Cells & Nuclei for Chromium Fixed RNA Profiling (CG000478).

The Fixed RNA Gene Expression library was generated as described in the Chromium Fixed RNA Profiling for Multiplexed Samples User Guide (CG000527) using the Chromium X. Samples were run as a 4-sample multiplexed experiment, in which each sample was hybridized with a unique Probe Barcode in a separate hybridization reaction. After hybridization, samples were pooled in equal proportions, washed, and then run in a single GEM lane. The resulting library was sequenced on an Illumina NovaSeq6000 with approximately 92k read pairs per cell.

• Total 26,870 cells detected (Bladder: 8,371; Breast: 5,880; Ovary: 5,758; Kidney: 6,861)
• Paired-end, dual indexing: 28 cycles Read 1, 10 cycles i7, 10 cycles i5, 90 cycles Read 2

This dataset is licensed under the Creative Commons Attribution license.