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10k Mouse Splenocytes, Chromium GEM-X Single Cell 5'

Universal 5' Gene Expression dataset analyzed using Cell Ranger 8.0.0

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Splenocytes were obtained from an adult C57/BL6 mouse spleen.

Gene Expression libraries were generated as described in the Chromium GEM-X Single Cell 5' Reagent Kits v3 User Guide (CG000733) and sequenced on an Illumina NovaSeq 6000 with approximately 80,000 read pairs per cell.

Paired-end, dual indexing:

  • 28 cycles Read 1
  • 10 cycles i7
  • 10 cycles i5
  • 90 cycles Read 2

The cellranger multi pipeline in Cell Ranger v8.0.0 was used to analyze libraries.

This dataset is licensed under the Creative Commons Attribution license.