10k Adult Mouse Brain Nuclei Isolated with Chromium Nuclei Isolation Kit, Chromium GEM-X Single Cell 3'
Universal 3' Gene Expression dataset analyzed using Cell Ranger 8.0.0
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Nuclei were isolated from ~25 mg of fresh frozen C57/Bl6 adult mouse brain purchased from BioIVT following the Chromium Nuclei Isolation Reagent Kits Sample Prep User Guide (CG000505).
Gene Expression libraries were generated as described in the Chromium GEM-X Single Cell 3' Reagent Kits v4 User Guide (CG000731) and sequenced on an Illumina NovaSeq6000 with approximately 50,000 read pairs per cell.
Paired-end, dual indexing:
- 28 cycles Read 1
- 10 cycles i7
- 10 cycles i5
- 90 cycles Read 2
The cellranger multi pipeline in Cell Ranger v8.0.0 was used to analyze libraries.
This dataset is licensed under the Creative Commons Attribution license.