10k Human PBMCs, Multiome v1.0, Chromium X

Human peripheral blood mononuclear cells (PBMCs) of a healthy male donor aged 30-35 were obtained by 10x Genomics from AllCells. Nuclei were isolated using the demonstrated protocol Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing (CG000365).

Gene Expression and ATAC libraries were generated from ~16,100 transposed nuclei (10,974 nuclei recovered) as described in the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide (CG000338 Rev E) using the Chromium X and sequenced on an Illumina NovaSeq 6000.

ATAC libraries were sequenced to ~55k read pairs per cell.

• 50 cycles Read 1
• 8 cycles i7 (sample index)
• 24 cycles i5 (10x barcode)
• 49 cycles Read 2

Gene Expression libraries were sequenced to ~50k reads per cell.

• 28 cycles Read 1 (16 bp barcode, 12 bp UMI)
• 10 cycles i7 (sample index)
• 10 cycles i5 (sample index)
• 90 cycles Read 2 (transcript)

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.