10k 1:1 Mixture of Raji and Jurkat Cells Multiplexed, 2 CMOs

Universal 3' Gene Expression dataset analyzed using Cell Ranger 6.0.0

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

Jurkat and Raji cell lines obtained by 10x Genomics from ATCC were multiplexed at equal proportions with 1 CMO per cell line, resulting in a pooled sample labeled with 2 CMOs.

Libraries were prepared following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Multiplexing User Guide (CG000388) and sequenced on Illumina NovaSeq 6000.

Single Cell 3’ Cell Multiplexing v3.1 Dual Index Library

Sequencing Depth: 28,266 read pairs per cell
Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI); i5 index: 10 cycles (sample index); i7 index: 10 cycles (sample index); Read 2: 90 cycles (transcript)

Pooled Multiplexed Sample - Key Metrics

Estimated Number of Cells: 13,615
Cells Assigned to a Sample: 11,070

Individual Multiplexed Samples - Key Metrics

Metric	Jurkat	Raji
Cells Assigned to a Sample	5,875	5,195
Median Genes per Cell	3,682	3,081
Median UMI Counts per Cell	11,181	10,478

This dataset is licensed under the Creative Commons Attribution license.