For imaging hardware, general image acquisition and analysis guidelines, and example images

Imaging

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Spatial Gene and Protein Expression protocols

Experimental Design

Deparaffinize, decrosslink, and stain tissue

Find a list of probes targeting protein-coding genes in human transcriptome for the Spatial Gene and Protein Expression assay

Find a list of proteins detected by the human protein panel for the Spatial Gene and Protein Expression assay

Construct sequencing-ready Visium spatial libraries from your FFPE tissue sections

Refer to instrument-specific documents for seamless instrument operation

Sequence your Spatial Gene and Protein Expression libraries as per our provided recommendations

Analyze your Visium spatial data using our pipelines and visualization tools.