In whole transcriptome single cell data from Chromium Single Cell 3’ and 5’ products, reads primarily map to exonic regions derived from mature spliced mRNA transcripts. However, reads may also map entirely or partially to intronic regions (Figure 1). This Technical Note evaluates the effects of counting intronic reads for Single Cell Gene Expression cellular data.
Interpreting Single Cell Gene Expression Data With and Without Intronic Reads
Technical Note, CG000554
CG000554_Interpreting_SingleCellGEX_with_introns_RevA.pdf
- Document Type
- Technical Note
- Last Modified
- June 21, 2022