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Provides safety information for Automated Chromium Single Cell 3' v3.1 assay reagents.

Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits Safety Data Sheets (v3.1 Chemistry)

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

Chromium Universal 5' Gene Expression allows analysis of full-length, paired B-cell or T-cell receptor (BCR/TCR) sequences, surface protein expression, antigen specificity, and 5’ gene expression, all from a single cell. Scale up with a high-throughput solution, or focus on key genes with targeted gene expression panels.

Plan ahead by referring to the list of equipment, kits, and reagents needed for Single Cell Immune Profiling.

Experimental Design

Experimental Design & Planning

Prepare optimal single cell/nuclei suspension by following our Demonstrated Protocols.

Sample Prep

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Use pre-designed or custom panels for targeted gene expression, followed by sequencing and data analysis.

Targeted

Targeted Gene Expression (Optional)

Sequence your single cell library as per our provided recommendations.

Sequencing

Analyze your single cell data using our pipelines, visualization, and Cloud Analysis tools.

Software Analysis

Chromium X シリーズ

Universal 5' 遺伝子発現

Overview

User Guides

Workflow Documentation

Additional Applications

Datasets

Videos

Publications

Universal 5' Gene Expression

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Chromium Universal 5' Gene Expression allows analysis of full-length, paired B-cell or T-cell receptor (BCR/TCR) sequences, surface protein expression, and 5’ gene expression, all from a single cell.

What is Universal 5' Gene Expression?

Measure single cell gene expression, cell surface protein expression, and optionally antigen specificity simultaneously.

Cell Surface Protein

Assess the effects of CRISPR perturbations alongside single cell gene expression.

CRISPR Screening

Enables molecular profiling of 2,000–20,000 cells/sample with simultaneous detection of full length, paired immune receptor sequences, gene and protein expression, and antigen specificity.

High Throughput (HT)

Map antigens to discover novel antigen-specific Ig or T-cell receptors.

Barcode Enabled Antigen Mapping (BEAM)

We provide resources from protocols, user guides, and free-to-use analysis tools.

Documentation

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

Chromium Next GEM Single Cell 5' v2 Dual Index

Chromium Next GEM Single Cell 5' HT v2 Dual Index

Single Cell 5' v2 Barcode Enabled Antigen Mapping (BEAM)

Find answers to common technical questions, from sample prep through data analysis, about Chromium single cell workflows.

Q&As

Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.

Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits Safety Data Sheets (v3.1 Chemistry)

SDS,

Module 1_Chromium Next GEM Automated Single Cell 3’ Library_Ed 1.pdf

SDS,

Module 2_Chromium Next GEM Automated Single Cell 3’ Library_Ed 1.pdf

SDS,

Module 3_Chromium Next GEM Automated Single Cell 3’ Library_Ed 1.pdf

SDS,

1000212_1000213_1000215_Sample_Index_Kits.pdf

SDS,

1000137_1000148_Gel Bead_Chromium Next GEM Automated Single Cell 3' Library_Ed 1.pdf

SDS,

1000136_1000146_Chromium Next GEM Chip G Automated Single Cell Kit_Ed 1.pdf

SDS,

2000048_Dynabeads_MyOne_SILANE_sds.pdf

SDS,

1000395_Chromium Next GEM Automated cDNA Module 1_Ed1.pdf

SDS,

1000396_Chromium Next GEM Automated cDNA Module 2_Ed1.pdf

SDS,

1000399_Chromium Automated LibraryModule 1_Ed1.pdf

SDS,

1000397_1000398_ChromiumNext GEM Automated cDNA Module 3_Ed1.pdf

SDS,

1000400_Chromium Automated LibraryModule 2_Ed1.pdf

SDS,

1000401_Chromium AutomatedLibrary Module 3_Ed1.pdf

SDS,

Gel_Bead_Chromium_Next_GEM_Automated_Single_Cell_3_Library_Ed2.pdf