Technical Note - Importance of Cell Stock Concentration for Accurate Target Cell Recovery

This Technical Note highlights cell stock concentration as one factor that can impact the accuracy of the final cell count and ultimately, the agreement between targeted and calculated cell recovery. Accurate counting and assessment of cell viability are required as part of the workflow outlined in the Single Cell Protocols and are essential to determine the number of cells loaded onto the microfluidic chip for single cell partitioning. Manual (e.g. hemocytometer) and automated (e.g. Countess® II Automated Cell Counter) counting methods are common techniques to determine cell concentrations. A number of factors that can affect the final cell count are discussed.

FOR USE WITH

• Chromium™ Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns PN-120237
• Chromium™ Single Cell 3’ Library & Gel Bead Kit v2, 4 rxns PN-120267
• Chromium™ Single Cell 5’ Library & Gel Bead Kit, 16 rxns PN-1000006
• Chromium™ Single Cell 5’ Library & Gel Bead Kit, 4 rxns PN-1000014
• Chromium™ Single Cell V(D)J Enrichment Kit, Human T Cell, 96 rxns PN-1000005
• Chromium™ Single Cell A Chip Kit, 48 rxns PN-120236
• Chromium™ Single Cell A Chip Kit, 16 rxns PN-1000009
• Chromium™ i7 Multiplex Kit, 96 rxns PN-120262