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GEM-X Flex
Whole transcriptome gene expression, with ultimate flexibility in sample prep and the ability to fix, batch, and run samples on your schedule We recommend Flex Gene Expression in experiments that:
Profile up to 384 samples a week with 96-well sample multiplexing
Require flexible sample batching and the ability to run partial plates
Use archival or lower-quality samples
GEM-X Universal 3'
A versatile solution providing 3' gene expression with options for multiomic analysis We recommend Universal 3' in experiments that...
Require full-length transcript readouts, with or without simultaneous protein expression
Only require gene expression, or may use ATAC in the future
Have lower cell input requirements
GEM-X Universal 5'
Comprehensive analysis of immune diversity, combining 5' gene expression with multiomic profiling We recommend Universal 5' in experiments that...
Require full length transcript readouts, with or without simultaneous protein expression
Require or may use CRISPR and TCR/BCR sequencing in the future
Have lower cell input requirements
Primary capability
Probe-based gene expression
Analyze gene expression using pre-designed probes with the option to add custom probes
3' gene expression
Detect and quantify RNA molecules near the 3' end of genes
5' gene expression
Detect and quantify RNA molecules near the 5' end of genes
Multiomic capabilities
Cell surface protein
Identify surface protein markers to categorize cell types, pinpoint biomarkers, and explore cell signaling and interactions
CRISPR screening
Assess effects of CRISPR perturbations at single cell resolution with direct capture of cellular sgRNAs and changes in gene expression
Intracellular protein
Achieve detection of proteins throughout the entire cell, including nuclear, cytosolic, and secreted proteins
Cell surface protein
Identify surface protein markers to categorize cell types, pinpoint biomarkers, and explore cell signaling and interactions
Cell surface protein
Identify surface protein markers to categorize cell types, pinpoint biomarkers, and explore cell signaling and interactions
CRISPR screening
Assess effects of CRISPR perturbations at single cell resolution with direct capture of cellular sgRNAs and changes in gene expression
TCR/BCR sequencing
Understand clonal expansion and diversity by capturing full-length, paired B-cell and T-cell receptors
Antigen specificity
Interrogate the binding of antigens to immune cell receptors to fully characterize the adaptive immune response.
Compatible species
Human
Mouse
Rat
Non-human primate
Marine
Plant
Insect
And more
Human
Mouse
Rat (except with TCR/BCR)
Non-human primate (Except with TCR/BCR)
And more
Compatible sample types
Cell suspension
Nuclei suspension
Fresh tissue
Frozen tissue
FFPE tissue
PFA-fixed tissue
Flow-sorted cells
Fixed whole blood
Organoids
Cell suspension
Nuclei suspension
Fresh tissue
Frozen tissue
Fixed human PBMCs
Flow-sorted cells
Organoids
Cell suspension
Nuclei suspension
Fresh tissue
Frozen tissue (gene expression only)
Fixed human PBMCs
Flow-sorted cells
Organoids
Resolution
Whole cell/Nucleus
Cells or nuclei are partitioned into GEM Gel Beads that uniquely barcode each, enabling single cell resolution
Whole cell/Nucleus
Cells or nuclei are partitioned into GEM Gel Beads that uniquely barcode each, enabling single cell resolution
Whole cell/Nucleus
Cells or nuclei are partitioned into GEM Gel Beads that uniquely barcode each, enabling single cell resolution
Run configuration options
Compatible instruments
Chromium iX and X
Access our core assays and capabilities with the iX or take advantage of our full single cell portfolio with the Chromium X.
Chromium Xo, iX, and X
Perform 3' gene expression with the low-cost Xo instrument, access our core assays and capabilities with the iX, or take advantage of our full single cell portfolio with the Chromium X.
Chromium iX and X
Access our core assays and capabilities with the iX or take advantage of our full single cell portfolio with the Chromium X.
Included data processing & analysis software
Cell Ranger
Cell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data on-prem.
Cloud Analysis
A platform for running 10x analysis pipelines on optimized cloud clusters for a quick and easy path to results.
Cell Ranger
Cell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data on-prem.
Cloud Analysis
A platform for running 10x analysis pipelines on optimized cloud clusters for a quick and easy path to results.
Cell Ranger
Cell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data on-prem.
Cloud Analysis
A platform for running 10x analysis pipelines on optimized cloud clusters for a quick and easy path to results.
Included interactive visualization tools
Loupe Browser
Visualize and interpret Chromium single cell gene expression data with Loupe Browser, a visualization tool that enables identification of gene expression patterns and cell clusters.
Loupe Browser
Visualize and interpret Chromium single cell gene expression data with Loupe Browser, a visualization tool that enables identification of gene expression patterns and cell clusters.
Loupe Browser
Interactively explore clonotype information and repertoire diversity alongside gene expression data in Loupe Browser.
Loupe V(D)J Browser
Investigate clonotypes and examine V(D)J transcripts with this interactive visualization desktop software.